人卵巢癌细胞
细胞名称：NIH/3T3小鼠胚胎细胞
描述：NIH-OVCAR-3细胞由T.C.Hamilton在1982年建系。 取材于患进行性卵巢腺癌病人的恶性腹水。 与107细胞联合皮下接种５只裸鼠21天后全部成瘤。 NIH-OVCAR-3是研究卵巢癌药物抗性的一个合适的模型系统且由于存在激素受体，这对于激素治疗的评估或许是有用的。
动物种别：人
性别：女
组织来源：卵巢腺癌
形态：上皮细胞
培养基和添加剂：RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)，90%；添加0.01毫克/毫升牛胰岛素，80%；优质胎牛血清，20%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制：A。仅供研究之用。
REFERENCE：Hamilton TC , et al. Characterization of a human ovarian carcinoma cell line (NIH:OVCAR-3) with androgen and estrogen receptors. Cancer Res. 43: 5379-5389, 1983. PubMed: 6604576 Hamilton TC , et al. Induction of progesterone receptor with 17beta-estradiol in human ovarian cancer. J. Clin.。 Endocrinol. Metab. 59: 561-563, 1984. PubMed: 6746867 Rogan AM , et al. Reversal of adriamycin resistance by verapamil in human ovarian cancer. Science 224: 994-996, 1984. PubMed: 6372095 Hamilton TC , et al. Characterization of a xenograft model of human ovarian carcinoma which produces ascites and intraabdominal carcinomatosis in mice. Cancer Res. 44: 5286-5290, 1984. PubMed: 6333272 Green JA , et al. Potentiation of melphalan cytotoxicity in human ovarian cancer cell lines by glutathione depletion. Cancer Res. 44: 5427-5431, 1984. PubMed: 6488194 Caffrey PB , Frenkel GD . Selenite cytotoxicity in drug resistant and nonresistant human ovarian tumor cells. Cancer Res. 52: 4812-4816, 1992. PubMed: 1511444 Hamilton TC , et al. Experimental model systems of ovarian cancer: applications to the design and evaluation of new treatment approaches. Semin. Oncol. 11: 285-298, 1984. PubMed: 6385258 Godwin AK , et al. High resistance to cisplatin in human ovarian cancer cell lines is associated with marked increase of glutathione synthesis. Proc. Natl. Acad. Sci. USA 89: 3070-3074, 1992. PubMed: 1348364 Chang K , Pastan I . Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591 Omelyanenko V , et al. HPMA copolymer-anticancer drug-OV-TL16 antibody conjugates. II. Processing in epithelial ovarian carcinoma cells in vitro. Int. J. Cancer 75: 600-608, 1998.
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