BT-474 [BT474]人乳腺导管癌细胞
描述:E. Lasfargues 和 W.G. Coutinho从一个实心的乳腺浸润性导管癌中分离到了BT-474细胞株。
动物种别:人
性别:女
组织来源:乳腺导管癌
形态:上皮细胞
培养基和添加剂:DMEM培养基(GIBCO,货号12800017,添加NaHCO3 1.5g/L),90%;胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。
供应限制:A。仅供研究之用。
REFERENCE:Lasfargues EY , et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572 22660: Lasfargues EY , et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035 32275: Littlewood-Evans AJ , et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997.
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