293T人胚肾细胞
描述：293细胞株插入SV40 T-抗原的温度敏感基因后产生的高转染效率的衍生株称为293T。
动物种别：人
性别：***
组织来源：肾
形态：上皮细胞
培养基和添加剂：DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。
供应限制：A。仅供研究之用。
REFERENCE：Sena-Esteves M , et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999. PubMed: 10559361 Pensiero M , et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999 Pensiero M , et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 6,329,199 dated Dec 11 2001 Pear WS , et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960
中文名称：人胚肾细胞
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