Dihydrozeatin O-Glucoside can be used as analyte in analytical study and preparation processes for profiling of phytohormones and their major metabolites in rice using binary solid-phase extraction and liquid chromatography triple quadrupole mass spectrometry. It can also be utilized in biological study. Total amount of endogenous cytokinins was higher in shoots than in roots of Centaurium erythraea where the inactive or weakly active N-glucosides was predominate in shoots and roots.