(3β)-26,27-Dinorcholest-5-en-24-yne-3,20-diol-d4 is the isotope labelled analog of (3β)-26,27-Dinorcholest-5-en-24-yne-3,20-diol. (3β)-26,27-Dinorcholest-5-en-24-yne-3,20-diol is a byproduct of cholesterol (C432501) oxidation and a potential agonists of the hedgehog signaling pathway.