(E)-tert-Butyl 6-Chlorohex-2-enoate is an intermediate for the synthesis of 3S-Hydroxy-N6,N6,N6-trimethyl-L-lysine Chloride (H977645), which is a metabolite in the biosynthesis of carnitine and acts as a biomarker in its analysis. Carnitine is a compound that enables intracellular transport of fatty acids.