2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole; 2-[2-(4-Ethoxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazinyl)benzimidazole; BXI 72; Bisbenzimide; HOE 33342; Ho 342; Hoechst H 33342; NSC 334072; 2'-(p-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole;
CAS号:
23491-52-3
分子式:
C₂₇H₂₈N₆O
分子量:
452.55
种类:
Research Tools; Materials; Dyes;
应用:
Hoechst 33342 is an A:T-specific DNA minor groove ligand that is a widely used fluorochrome for visualizing cellular DNA. Excitation of the dye occurs around 350 nm with an emission maxima around 461 nm. Hoechst-33342, unlike other Hoechst dyes, is more lipophillic due to an additional ethyl group. The compound has been observed to displace several known DNA intercalators. Adriamycin has been shown to disrupt the DNA dye update. The compound can be used in sorting living cells based on DNA content by FACS, in the visualization of chromatin distribution in living cells, to detect BrdU incorporation into cells, the visualization of fixed cells by microscopy, and to study the initial stages of apoptosis and cell-cycle distribution. In addition to Hoechst-33342's fluorescent capability, the
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